# Fatty Acid Synthesis (Citrate Shuttle)

**Fatty Acid Synthesis** is a biochemical pathway which uses the **Citrate Shuttle** to produce palmitate, a fatty acid, from other molecules.

First, **citrate** from the mitochondrial matrix is shuttled to the cytosol via the citrate shuttle. This citrate is then converted by **ATP citrate lyase** into **Acetyl CoA**.

Afterwards, Acetyl-CoA is turned into **Malonyl-CoA** via the [**Vitamin B7 (Biotin)**](/content/view/3765/index.html)-dependent **Acetyl-CoA carboxylase** enzyme.

Finally, this Malonyl-CoA is converted into **Palmitate**, a 16-C fatty acid at the end of this pathway.

Find Fatty Acid Synthesis and more [Biochemical Pathways](/content/browse#playlist-4028/index.html) among Pixorize's visual mnemonics for the USMLE Step 1 and NBME Shelf Exams.